NicAlert Cotinine Saliva Strip NicAlert determines degree of Nicotine smoke exposure by measuring cotinine in saliva. Nicotine, main drug in tobacco smoke, is processed by body into cotinine. (require saliva sample)

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NicAlert Instructions

The NicAlert™ System is formulated for use with human oral fluid specimens, saliva lang=en-us> or urine. These procedures cover the saliva method. Confirm the expiration date printed on the test package. Do not open the test package until you are ready to perform the saliva cotinine assay. If, necessary, bring the test pouch to room temperature . Insure that the person being tested has had nothing in their mouth for at least 5 minutes before sample collection. This includes water or other beverages (dilution) or food of any type (contamination).

1. Collect the oral fluid sample by using the funnel device and collection container provided. The test subject should spit into the funnel until the collection container is filled at least one third with saliva. Never insert the strip directly in the subject's mouth as the strip will not react properly.

2. Remove the funnel from the collection container and plug the larger open end with the stopper provided.

3. Lay the NicAlert™ test device on a dry flat surface with the numbered levels facing up.

4. Squeeze the collection container and apply the saliva sample to the absorbent cotton wick end of the test strip. Apply approximately 4-5 drops or until the wick is completely saturated waiting at least ten (10) seconds between each drop. Allow each drop to soak into the wick before applying the next drop. In the case of very viscous samples, you may need to add additional drops. apply the 4th drop on top of the 3rd drop when the 3rd drop has almost sunk all the way into the sample pad. Add enough saliva sample that visible sample migration across the test result panel begins.

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