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NicAlert™ is the world's first oral fluid (saliva) based, point of contact, lateral flow immunoassay for Nicotine (Tobacco) use or abuse. Simple and discreet nicotine or smoking status testing without the inconvenience, embarrassment or biological hazard risks associated with urine specimen collection and handling. Completely eliminates the possibility of urine sample contamination, adulteration, substitution or manipulation. Semi-quantitative results using saliva in approximately 15 minutes. Extremely sensitive to cotinine, the principle metabolite of nicotine, the NicAlert™ test will detect 6 ranges of cotinine concentrations from 0 ng/ml through 2000+ ng/ml.
Principle
Incorporating a newly developed and highly sensitive chromo graphic lateral flow strip type testing device along with a special collection device for human saliva samples, the NicAlert™ nicotine test is a self-contained, ready to use, point of contact drug testing device that detects the presence of cotinine, the principal metabolite of nicotine in human saliva samples. Using the preferred lateral flow immunoassay technology for this method of test screening, the NicAlert™ test requires no special training to complete and semi-quantitative results are visibly obtained within minutes.Oral Fluid Assay System For NICOTINE USE
The NicAlert™ forensic nicotine testing system detects cotinine, the principle metabolite of nicotine, one of the most addictive drugs of abuse using a saliva sample. Because urine collection is not necessary, there are certain advantages using the NicAlert™ system for on-site nicotine use or smoking status screening. Additionally, because the test subject is present for the entire sample collection and testing process, the possibility of sample adulteration and/or substitution is eliminated. The NicAlert™ test is highly sensitive and will detect even the smallest concentrations of cotinine. Each test strip is divided into six (6) reactive chromo-graphic (color change) levels of cotinine detection. Results are semi-quantitative based on the following detection Table:
| Table 1. Cotinine Equivalents for each Level | |
| Level on Strip | Cotinine Equivalent |
| 0 | 1-10 ng/ml |
| 1 | 10-30 ng/ml |
| 2 | 30-100 ng/ml |
| 3 | 100-200 ng/ml |
| 4 | 200-500 ng/ml |
| 5 | 500-2000 ng/ml |
| 6 | 2000+ ng/ml |
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