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Rules, Procedures, and Common Mistakes
pertaining to handling blood for
cholesterol testing to get optimal accuracy
| Rules for testing cholesterol | |
| Rule | Description |
| 1. | Testing cholesterol is NOT the
same as testing glucose Cholesterol testing requires more precise method of collecting blood |
| 2. | Amount of blood and way it is obtained is critical for optimal accuracy for cholesterol testing |
| 3. | Test accuracy can degrade when exposed to light and/or humidity over time; keep exposure under 1 minute |
| 4. | Test accuracy can be improved by taking the time to actually read the CardioChek manual and this document |
| Procedures
for properly handling blood to do cholesterol testing that increases accuracy (organized in order of execution) |
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| Step | Description | Mistakes |
| 1. | Read CardioChek manual and this printout | rule #4 |
| 2. | Fast for 12 hours only if you will be testing triglycerides to get optimal accuracy | mistake #12 |
| 3. | Do physical exercise to get ‘blood pumping’ before lancing finger to get larger blood drop | mistake #4,5 |
| 4. | Thoroughly clean finger preferably with alcohol prep pad | mistake #2 |
| 5. | Use properly sized lancet to ensure large enough blood drop; avoid undersized lancets used only for testing glucose | rule #2 mistake #4,7 |
| 6. | Lance finger on the side of finger | mistake #8 |
| 7. | Use 2nd drop of blood from finger; wipe 1st drop away with cloth (very important) | mistake #1,2 |
| 8. | Use freely dangling blood drop | mistake #5,11 |
| 9. | Place capillary under freely dangling blood drop | mistake #5,11 |
| 10. | Use capillary filled with blood to indicator line | mistake #4 |
| 11. | Remove memo chip from vial and insert into top side of analyzer | mistake #6,9,10 |
| 12. | Remove test strip from vial and slide test strip into analyzer | mistake #6,9,10 |
| 13. | Apply blood to test strip within 1 minute after removing it from vial | mistake #6,9,10 |
| 14. | Keep other tests inside vial with sealed lid and only open lid to remove test for immediate use | mistake #6,9,10 |
| 15. | Drip blood being squeezed (or plunged) from capillary onto test strip without making contact | mistake 3,13,14 |
| Common Mistakes
made while handling blood to do cholesterol testing that reduce accuracy (ranked in order of importance) |
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| Mistake | Description |
| 1. | Don’t use 1st drop of blood; rather, remove 1st drop with a cloth then use 2nd drop |
| 2. | Don’t use `contaminated blood` from 1st drop, lancing tip of finger, or not properly cleaning finger |
| 3. | Don’t try to apply blood directly onto test strip without capillary; rather, always use a capillary |
| 4. | Don’t settle for too little blood in capillary whereby blood does not reach the indicator line |
| 5. | Don’t squeeze blood from finger; rather, massage top hand then work massage down |
| 6. | Don’t wait longer than 1 minute to apply blood from capillary to test strip |
| 7. | Don’t used an undersized (glucose) lancet because cholesterol testing requires more blood than glucose |
| 8. | Don’t lance the tip of finger and get `contaminated blood`; rather, lance side of finger |
| 9. | Don’t let test strips remain outside of vial for more than 1 minute before using it |
| 10. | Don’t let tests inside vial get exposed to light and humidity by leaving vial open with an unsealed lid |
| 11. | Don’t force blood in the capillary; rather, allow blood drop to tangle over capillary |
| 12. | Don’t eat for 12 hours if you will be testing triglycerides |
| 13. | Don’t let blood remain in capillary by failing to place finger over the ‘breath’ hole of capillary |
| 14. | Don’t let capillary make contact with test strip |
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