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TobacAlert Instructions

PLEASE READ THE INSTRUCTIONS CAREFULLY BEFORE USE

What are the uses of TobacAlert™?

TobacAlert™ allows you to assess your level of exposure to second-hand smoke. It is not intended for medical use.

TobacAlert™ measures cotinine in urine for the purpose of determining if you have been exposed to environmental tobacco smoke (second hand smoke) produced by cigarettes, pipes, cigars, etc. Within the past 2 to 3 days. The cutoff concentration for the TobacAlert™ test is 6 ng/mL. Smokers and users of tobacco products will also test positive.

Before You Begin

• Carefully read through the entire pamphlet.
• Do not use after the expiration date printed on the foil pouch.
• Do not open the foil pouch until you are ready to do the test.
• Have a watch, clock or timer available.
• Have a clean, leak-proof container available to collect your urine.

Instructions

1. Collect urine using any clean container available.
2. Tear the foil pouch at the notch and remove the strip.
3. Holding the blue end (handle end) of the strip, dip the strip in the urine sample container for 10 to 20 seconds. Dip the strip in the sample up to the level of the arrows on the strip.
4. Then put the strip on the reading card in the space provided for at least 20 minutes before reading the result. Do not place the strip anywhere else. Flush the urine collected and wash or discard the container.
5. Read the results at least 20 minutes after dipping. The reading is the LOWEST reddish line on the strip. Compare your strip with the examples shown on the reading card. Find the example that most closely matches your strip. If your result is "0" (negative), then you have not been exposed to second hand smoke within the past 48 hours. If your result is "1", then you have significant second hand smoke exposure. If there are no clear reddish lines on the strip, repeat the test from the beginning with a new strip. If the result is unclear to you and you think it could be either "0" or "1" and you cannot decide, repeat the test at another time.

Materials Provided

TobacAlert™ Strip: Each test strip is individually packaged in a sealed labeled plastic pouch.

Each lest strip is composed of the following:

1. 5mm X 90mm nitrocellulose impregnated with:
2. Mouse monoclonal antibodies reactive to cotinine, conjugated to colloidal gold particles.
3. Rabbit anti-mouse polyclonal antibodies reactive to mouse antibodies.
4. 1% Cobalt Blue 406 #2 dye.
5. Carboxycontinine, isopropyl carboxynorcotinine, carboxyphenylethylcotinine bromide.
6. 50mM sodium phosphate buffer pH 7.2, bulking agents, stabilizers.
7. Cotton pads, filters.

Materials Required

• A timer or clock.
• For urine sample collection a clean, leak-proof, disposable container (urine specimen container) is required.
• Forceps, tweezers, or gloves are preferred for holding the strip when dipping it into the urine sample.

NOTE: This test is for single use. Use within 10 minutes of opening the pouch. Discard after use. Discard any samples if contamination is suspected and obtain another sample. Do not consume or handle food or drink near the  TobacAlert™ strip or when performing the test. Test samples at room temperature. The TobacAlert™ strip should not he used on cloudy or pink urine samples. Do not use the test after the expiration date. Do not place the strip in your mouth. TobacAlert™ is not for medical use.

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