Step 1: Collect Semen

Semen may be collected by masturbation directly into the Liquefaction Cup or using the provided condom. Semen may also be collected by using the condom during intercourse. Avoid touching the inside of the Liquefaction Cup.

Step 2:

If a condom was used to collect your semen, transfer the semen from the condom to the Liquefaction Cup. Place the lid on the Liquefaction Cup and swirl gently at least 10 times. Wait at least 15 minutes but not longer than 12 hours to begin testing. Swirl the Liquefaction Cup before you begin testing.

Step 3:

Open resealable, foil pouch by tearing or cutting at notches. Remove contents. Place the FertilMARQ™ cassette on a flat surface with the wells facing up.

Step 4:

Swirl Liquefaction Cup before removing lid. Hold and squeeze the plastic dropper at its midpoint. Place the angled tip into the semen sample and release the pressure slowly. Semen will be drawn up into the dropper.

Step 5:

By gently squeezing the dropper, add ONE drop of SEMEN to the Test Well B.

Let the drop soak in for at least one minute.

Step 6:

Add TWO drops of BLUE solution to Test Well B.

Let the drops soak in for at least one minute.

Step 7:

Add TWO drops of CLEAR solution to Test Well B.

Let the drops soak in for at least one minute.

Read results within 5 minutes according to Step 8.

Step 8: Reading the Color Change

Compare the color in the Test Well B to the color in the Reference Well A.

Positive Test: A blue color as dark or darker than the reference color is positive (sperm concentration is equal to or above 20 million/mL).

Negative Test: A blue color lighter than the reference color is negative (sperm concentration is less than 20 million/mL). Record results on Test Results Form.

Step 9:

After completing the first test, return the cassette and solution bottles to the foil pouch and seal the pouch. Dispose of the used Liquefaction Cup and dropper.

Step 10:

Second Test: Repeat test at least 3 days but not more than 7 days after first test. Follow complete instructions, Steps 1-8, using Test Well D and Reference Well C.

Disposal: After completion of second test, dispose of the entire kit in the regular trash.