 |
Step
1: Collect Semen
Semen may be
collected by masturbation directly into the Liquefaction Cup or using
the provided condom. Semen may also be collected by using the condom
during intercourse. Avoid touching the inside of the Liquefaction Cup.
|
 |
Step
2:
If a condom was
used to collect your semen, transfer the semen from the condom to the
Liquefaction Cup. Place the lid on the Liquefaction Cup and swirl gently
at least 10 times. Wait at least 15 minutes but not longer than 12 hours
to begin testing. Swirl the Liquefaction Cup before you begin testing.
|
 |
Step
3:
Open resealable,
foil pouch by tearing or cutting at notches. Remove contents. Place the
FertilMARQ™ cassette on a flat surface with the wells facing up.
|
 |
Step
4:
Swirl
Liquefaction Cup before removing lid. Hold and squeeze the plastic
dropper at its midpoint. Place the angled tip into the semen sample and
release the pressure slowly. Semen will be drawn up into the dropper.
|
 |
Step
5:
By gently
squeezing the dropper, add ONE drop of SEMEN to the Test Well
B.
Let the drop soak
in for at least one minute.
|
 |
Step
6:
Add TWO
drops of BLUE solution to Test Well B.
Let the drops
soak in for at least one minute.
|
 |
Step
7:
Add TWO
drops of CLEAR solution to Test Well B.
Let the drops
soak in for at least one minute.
Read results
within 5 minutes according
to Step 8.
|
 |
Step
8: Reading the Color Change
Compare the color
in the Test Well B to the color in the Reference Well A.
Positive
Test: A blue color as dark or darker than the reference color
is positive (sperm concentration is equal to or above 20 million/mL).
Negative
Test:
A blue color lighter than the reference color is negative (sperm
concentration is less than 20 million/mL). Record results on Test
Results Form.
|
 |
Step
9:
After completing
the first test, return the cassette and solution bottles to the foil
pouch and seal the pouch. Dispose of the used Liquefaction Cup and
dropper.
|
 |
Step
10:
Second Test:
Repeat test at least 3 days but not more than 7 days after first test.
Follow complete instructions, Steps 1-8, using Test Well D and Reference
Well C.
Disposal:
After completion of second test, dispose of the entire kit in the
regular trash.
|