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Allow the test device, specimen, and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test from the sealed pouch and use it as soon as possible.
2. Remove the collector from the sealed pouch and insert the sponge end of the collector into the mouth. Actively swab the inside of the mouth and tongue to collect oral fluid for a total of 3 minutes until the sponge becomes fully saturated. Gentle pressing the sponge between the tongue and teeth will assist saturation. No hard spots should be felt on the sponge when saturated.
3. Remove the collector from the mouth. Place saturated oral fluid collector into chamber and press sponge fully against the strainer to collect oral fluid. Discard the collector. Snap the cap shut on the collection chamber.
4. Place the test device on a clean and level surface. Unscrew cap cover from the collection chamber. Invert the collection chamber and transfer 3 drops of oral fluid (approximately 100 µL) into each specimen well of the test device and start the timer. Avoid trapping air bubbles in the specimen well. Place screw cap on the collection chamber. See illustration below.
5. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not read results after 1 hour.
6. Apply the security seal over screw cap and send to the laboratory for confirmation if necessary.
INTERPRETATION OF RESULTS
(Please refer to the previous illustration)
NEGATIVE:* Two lines appear. One red line should be in the control region (C), and another apparent red or pink line adjacent should be in the test region (Drug/T). This negative result indicates that the drug concentration is below the detectable level.
*NOTE: The shade of red in the test line region (Drug/T) will vary, but it should be considered negative whenever there is even a faint pink line.
POSITIVE: One red line appears in the control region (C). No line appears in the test region (Drug/T). This positive result indicates that the drug concentration is above the detectable level.
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test panel. If the problem persists, discontinue using the lot immediately and contact the manufacturer.
QUALITY CONTROL
A procedural control is included in the test. A red line appearing in the control region (C) is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique.
Click here for Drug Testing Supplies such as test cups, transport vials, adulteration tests, and latex gloves and masks.
Click here for a book on how to treat teens with drug use problems.
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